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Title: Burning Cell
Ref: JS-0074
Author: Gaja Lesnicar Pucko
CRG Group: Multicellular Systems Biology
Size: 800 x 800
Description: Mesenchymal cell expressing membrane targeted EGFP.
This image was taken with a novel in ovo microscopy approach. We electroporated young chick embryo with pCAGGS-gpiEGFP vector to obtain a salt and pepper distribution of labelled cells. The egg was sealed with Teflon membrane that allowed gas exchange and embryo visualization and placed under the CLSM microscope. A 30 microns thick z-stack of 0,5 micron steps was taken. Z-stack was color coded for depth and maximum projected in Fiji. I like the false solitude of this cell – in reality it is surrounded by a multitude of unlabelled cells.
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