CRG/UPF Proteomics Unit

HEAD OF UNIT

OVERVIEW

The Proteomics Unit is a joint effort with the University Pompeu Fabra and it focuses on ultimate mass spectrometry technology for screening and targeted proteomics. Applications include identification of proteins and post-translational modifications, biomarker analysis, and quantitative proteomics using both stable-isotope-labeling and label-free quantitation approaches.

UNIQUE CAPABILITIES

The instruments available at the Proteomics Unit make it one of the best-equipped Units in Europe. They cover the most advanced techniques of the proteomics field, and are accessible to national and international scientists. The Proteomics Unit team has a broad experience in data acquisition and data analysis for proteomics applications and it has been involved in high-level training initiatives (e.g. EMBO Practical Course on Targeted Proteomics).

EQUIPMENT

• Liquid Chromatography
  • HPLC Proxeon 300
  • UPLC Proxeon 1000
  • UPLC Proxeon 1000
  • HPLC Agilent 1200
  • HPLC Agilent 1200
  • Eksigent Ultra Tempo II
  • Eksigent Ultra Tempo II
  • Eksigent 425
• Mass Spectrometry
  • Sciex QTRAP 5500
  • Sciex TRIPLE-TOF 5600+
  • Thermo Orbitrap Velos Pro
  • Thermo Orbitrap XL
  • Thermo Orbitrap Fusion Lumos

APPLICATIONS AND SERVICES

• Targeted proteomics
• Screening proteomics
• Phospho-peptide identification and quantitation
• Protein quantitation by label-free or chemical labelling (SILAC, iTRAQ) methods
• SWATH acquisition and data analysis
• Data analysis and interpretation