Advanced Light Microscopy Unit

HEAD OF UNIT

OVERVIEW

The Advanced Light Microscopy Unit provides researchers at CRG/PRBB and external organizations with an outstanding range of high-end instruments and covers the entire spectrum of advanced light microscopy applications, including a recent focus on super-resolution microscopy. Several confocal and wide-field systems offer configurations for multicolor, multiphoton, single-molecule, in vivo time-lapse, and automated high-speed imaging.

UNIQUE CAPABILITIES

It is currently one of the best-equipped microscopy units in Europe, frequent beta-test and demo site and several of its instruments are technology flagships in Spain.

EQUIPMENT

• Super-resolution microscopy: Leica CW-STED, Leica GSDIM
• Single beam laser-scanning confocal microscopy: 4 systems (2 multiphoton microscopes, time-correlated single photon counting detectors)
• Andor Revolution XD spinning disk confocal microscope
• Zeiss Cell Observer HS wide-field microscope
• ImageXpress Screening Microscope w. transmission option
• Pulsed UV Laser ablation system
• Fluorescence Macro Zoom Microscope with ColorCCD
• Processing cluster + 2 processing workstations

APPLICATIONS AND SERVICES

• Super-resolution microscopy (STED, GSD/dSTORM)
• Two-photon and confocal microscopy
• Multi-position imaging
• Fluorescence (lifetime) correlation spectroscopy (FCS/FLCS)
• Fluorescence lifetime imaging (FLIM)
• Total internal reflection fluorescence (TIRF)
• Single molecule applications
• Laser ablation/wounding
• High content screening (HCS)
• Environmental control for in vivo imaging
• Automated widefield imaging
• Image processing and analysis