Research and Interests
Dr. Beato is interested in understanding how eukaryotic cells respond to external signals, in particular how signaling by steroid hormones is transduced to the nucleus to modulate gene expression. For this, his group is studying how the topological information that determines the position of nucleosomes and the general organization of chromatin in the cell nucleus modulates the hormonal response. They also try to use this knowledge to decipher the role of steroid hormones in breast and endometrial cancer cell proliferation and differentiation.
Expertise and Capabilities
Beato’s group uses a combination of model reporter systems and genome wide studies, together with other forefront “omics” techniques and high-resolution microscopy to explore how kinases and other relevant enzymes affect chromatin structure and dynamics via functional interactions with chromatin remodeling complexes. The group is specialized in techniques for studying changes in the 3D structure of the human genome - from nucleosomes to loops and topological associating domains - and how the energy required for these processes is generated.
The group uses breast and other cancer cell lines as model systems, and validates their results in vivo using mice.
Importantly, the group has identified a number of enzymes relevant for tumor proliferation and differentiation, which could serve as potential therapeutic new targets, and they are interested in screening for inhibitors using simple in vitro assays based on the wild type and mutant recombinant proteins.
To know more about the group, please click here.