Gian Gaetano Tartaglia

Research and Interests

Dr. Tartaglia investigates the role played by RNA molecules in regulatory networks controlling protein production. More specifically, he is interested in understanding the conditions under which specific cellular pathways become aberrant and give rise to pathologies. His aim is to manipulate the regulatory networks in order to prevent the formation of toxic aggregates.

His current research focuses on associations of coding/non-coding RNAs with proteins involved in:

  • Transcriptional and translational regulation (e.g., X-chromosome inactivation, differentiation processes);
  • Neurodegenerative diseases (e.g., Parkinson’s a-synuclein, Alzheimer’s disease amyloid protein APP, TDP-43 and FUS). 

Expertise and Capabilities

Tartaglia’s group has developed a number of advanced computational tools for in silico prediction of alterations in protein-protein and protein-gene interactions that impair cell viability:

  • Protein engineering (ccSOL);
  • Aggregation and amyloidosis (Zyggregator);
  • Protein-RNA interactions (catRAPID);
  • De novo discovery of regulatory motifs in RNA/DNA binding proteins (SeAMotE).

The group has the expertise and resources to validate the computational findings in vitro, using state of the art experimental techniques such as:

  • Electrophoretic mobility shift assays (EMSA);
  • RNA-binding protein immunopurification (RIP);
  • Thioflavin T fluorescence spectroscopy;
  • Size exclusion chromatography (SEC);
  • Transmission electron microscopy (TEM).

 

Additional Information

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